Combining hotcompressed water and ball milling ...
15/04/2008 · To prepare combined hotcompressed water (HCW) and ball milling (BM) residues, HCW residues were milled for 20 minutes with pulverisette 7 as described in materials and methods. The HCWBM residue (5%) was hydrolyzed with an enzyme cocktail (4 FPU/g substrate) for 72 hours.