Palmitate induces fat accumulation by activating C/EBPβ ...
To measure the nuclear C/EBPβ protein level, nuclear protein extracts were isolated from HepG2 cells using NEPER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, United States). Meanwhile, HepG2 cells were harvested and lysed with icecold RIPA lysis buffer containing protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) and proteins were ...